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3D immunofluorescence staining of the implant surface harvested from young and aged mice. ( A ) After 1 week post‐implantation, the implants were polished, fixed, and stained using alexa fluor plus 555 donkey anti‐mouse IgG secondary antibody to detect <t>CD3</t> (green) and DAPI to label nuclei (blue) (10×, z stacks). The white dashed lines represent the outline of the implant. ( B ) Confocal imaging and image orientation. The red frames indicate the image region.
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3D immunofluorescence staining of the implant surface harvested from young and aged mice. ( A ) After 1 week post‐implantation, the implants were polished, fixed, and stained using alexa fluor plus 555 donkey anti‐mouse IgG secondary antibody to detect <t>CD3</t> (green) and DAPI to label nuclei (blue) (10×, z stacks). The white dashed lines represent the outline of the implant. ( B ) Confocal imaging and image orientation. The red frames indicate the image region.
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3D immunofluorescence staining of the implant surface harvested from young and aged mice. ( A ) After 1 week post‐implantation, the implants were polished, fixed, and stained using alexa fluor plus 555 donkey anti‐mouse IgG secondary antibody to detect <t>CD3</t> (green) and DAPI to label nuclei (blue) (10×, z stacks). The white dashed lines represent the outline of the implant. ( B ) Confocal imaging and image orientation. The red frames indicate the image region.
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3D immunofluorescence staining of the implant surface harvested from young and aged mice. ( A ) After 1 week post‐implantation, the implants were polished, fixed, and stained using alexa fluor plus 555 donkey anti‐mouse IgG secondary antibody to detect <t>CD3</t> (green) and DAPI to label nuclei (blue) (10×, z stacks). The white dashed lines represent the outline of the implant. ( B ) Confocal imaging and image orientation. The red frames indicate the image region.
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Average 90 stars, based on 1 article reviews
lipid peroxidation assay kit #43674 - by Bioz Stars, 2026-03
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lipid peroxidation assay kit (#43674)  (Millipore)
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3D immunofluorescence staining of the implant surface harvested from young and aged mice. ( A ) After 1 week post‐implantation, the implants were polished, fixed, and stained using alexa fluor plus 555 donkey anti‐mouse IgG secondary antibody to detect <t>CD3</t> (green) and DAPI to label nuclei (blue) (10×, z stacks). The white dashed lines represent the outline of the implant. ( B ) Confocal imaging and image orientation. The red frames indicate the image region.
Lipid Peroxidation Assay Kit (#43674), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipid peroxidation assay kit (#43674)/product/Millipore
Average 90 stars, based on 1 article reviews
lipid peroxidation assay kit (#43674) - by Bioz Stars, 2026-03
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  Buy from Supplier

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3D immunofluorescence staining of the implant surface harvested from young and aged mice. ( A ) After 1 week post‐implantation, the implants were polished, fixed, and stained using alexa fluor plus 555 donkey anti‐mouse IgG secondary antibody to detect CD3 (green) and DAPI to label nuclei (blue) (10×, z stacks). The white dashed lines represent the outline of the implant. ( B ) Confocal imaging and image orientation. The red frames indicate the image region.

Journal: JBMR Plus

Article Title: RNA ‐ seq Analysis of Peri‐Implant Tissue Shows Differences in Immune, Notch, Wnt, and Angiogenesis Pathways in Aged Versus Young Mice

doi: 10.1002/jbm4.10535

Figure Lengend Snippet: 3D immunofluorescence staining of the implant surface harvested from young and aged mice. ( A ) After 1 week post‐implantation, the implants were polished, fixed, and stained using alexa fluor plus 555 donkey anti‐mouse IgG secondary antibody to detect CD3 (green) and DAPI to label nuclei (blue) (10×, z stacks). The white dashed lines represent the outline of the implant. ( B ) Confocal imaging and image orientation. The red frames indicate the image region.

Article Snippet: Subsequently, the primary antibody, CD3 (NBP2‐43674, Novus Biologicals, Littleton, CO, USA), was separately diluted in 5% donkey serum (1:100) and incubated overnight at 4°C.

Techniques: Immunofluorescence, Staining, Imaging